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Category:Indian films
Category:1987 films
Category:1980s Marathi-language films
Category:Indian comedy films
Category:Marathi remakes of Malayalam filmsThe purpose of this project is to investigate the subcellular components that regulate actin cytoskeleton dynamics and structure, as well as their relationships to cell morphology and motility. The approach we have taken is to elucidate the cell biology of actin regulatory proteins using Drosophila as a model. We have used a combination of Drosophila genetics, molecular and cell biology, and quantitative light microscopy to study the cell biological functions of two regulatory proteins that are important for both lamellipodial assembly and function, Cora and VASP. We have determined that loss of either protein results in a specific defect in lamellipodial formation, suggesting they may have separate functions. Using Drosophila genetics we have also identified several genes that appear to play important roles in lamellipodial function and shape. We are currently investigating the function of these genes in cell biological assays in order to identify the molecules involved. We have also collaborated with the laboratory of Dr. Greg Jeffery to study the effect of Drosophila p53 on actin in cells. We have used Drosophila genetics to identify that the Drosophila DNA damage inducible protein (Ddp) has functions similar to human p53 and, using a variety of cell biological assays, we have found that Ddp reduces cell motility and promotes cellular adhesion, both of which are opposite effects on cell motility to those of p53. This work is now being extended by Dr. Jeffery. Current studies are focused on trying to identify molecules that are involved in these effects of Ddp. We are also continuing studies on the subcellular distribution and regulation of actin at the leading edge of migrating cells. The formation of the leading edge during the migration of cells is one of the most dramatic and fascinating examples of cell biology. We are using Drosophila genetics to investigate the mechanisms regulating the formation and stabilization of leading edges. One recent success has been the finding that two genes, Enabled and Shotgun, are involved in a pathway that is required for the formation and stabilization of the leading edge. To complete this project we have recently found that a third gene, the gene Wiskott-Aldrich Syndrome, is also required in this pathway. We have
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